Journal: Journal of Molecular Endocrinology

Article Title: Coexpression of Wilms’ tumor suppressor 1 (WT1) and androgen receptor (AR) in the genital tract of human male embryos and regulation of AR promoter activity by WT1

doi: 10.1677/jme-06-0020

Figure Lengend Snippet: Figure 1 The expression of WT1 and AR in the external genitalia at 7 weeks SD. WT1 (A) and AR (B) were expressed in mesenchymal cells surrounding the urogenital sinus (UGS). AR expression was visible in a ubiquitous pattern in the genital tubercle (B). The boxes I and II represent the area of WT1 and AR coexpression shown by immunofluorescence. The images Ia, IIa, Ib, and IIb show immunofluorescent staining of WT1 (red) and AR (green) respectively. Merge images (Ic and IIc) depict WT1 and AR coexpression (yellow) in the mesenchymal cells surrounding the UGS. Magnification: A and B !2.5 and Ia–IIc !40.

Article Snippet: The sections were incubated with 4 mg/ml rabbit polyclonal anti-WT1 antibody (C-19, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or 8 mg/ml rabbit polyclonal antiAR antibody (N-20, Santa Cruz Biotechnology) for 1 h at room temperature.

Techniques: Expressing, Staining

Journal: Journal of Molecular Endocrinology

Article Title: Coexpression of Wilms’ tumor suppressor 1 (WT1) and androgen receptor (AR) in the genital tract of human male embryos and regulation of AR promoter activity by WT1

doi: 10.1677/jme-06-0020

Figure Lengend Snippet: Figure 2 The expression of WT1 and AR in the internal genitalia at 7 and 13 weeks SD. In the mesonephros, WT1 and AR were coexpressed in mesenchymal cells (m) and the Mu¨llerian duct epithelium (Md) (A). The AR was also expressed strongly in Wolffian duct epithelium (Wd) (B). In the epididymis, WT1 and AR were coexpressed in interstitial cells (i). In addition, the AR was expressed strongly in tubular epithelium (t) (D,E). In the vas deferens (vd), WT1 and AR were coexpressed in interstitial cells surrounding the duct (i) and AR staining was strong positive in ductal epithelium (G, H). In the gubernaculum testis (gu), WT1 and AR staining was visible in single cells (J, K). (C), (F), (I), and (L) represent negative controls respectively. (M) and (N) represent WT1 (red) and AR (green) expression in the gubernaculum testis by immunofluorescence respectively. Coexpression of WT1 and AR (yellow) is shown by merge images of (M) and (N). (O) Magnification: A–L!10 and M–O !40.

Article Snippet: The sections were incubated with 4 mg/ml rabbit polyclonal anti-WT1 antibody (C-19, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or 8 mg/ml rabbit polyclonal antiAR antibody (N-20, Santa Cruz Biotechnology) for 1 h at room temperature.

Techniques: Expressing, Staining

Journal: Journal of Molecular Endocrinology

Article Title: Coexpression of Wilms’ tumor suppressor 1 (WT1) and androgen receptor (AR) in the genital tract of human male embryos and regulation of AR promoter activity by WT1

doi: 10.1677/jme-06-0020

Figure Lengend Snippet: Figure 3 Transcriptional regulation of the AR gene by WT1 in vitro. (A) Schematic representation of the promoter construct, which was used in this study. K352 and C427 represent the position of putative WT1-binding sites (according to Shimamura et al. 1997). (B) Modification of AR gene transcription by increasing quantities of WT1 (50, 100, 250, and 500 ng) in CV1 cells. (C) Modification of the AR gene transcription by WT1 (500 ng) in different cell lines (CV1, Hela, LNCaP, and T293). The relative luciferase activity (RLU) of the empty vector pCMV was set at 1. Results are given as relative activation of the reporter by the expression constructs when compared with the empty vector (pCMV). All values represent the means of three separate transfection experiments (GS.E.M).

Article Snippet: The sections were incubated with 4 mg/ml rabbit polyclonal anti-WT1 antibody (C-19, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or 8 mg/ml rabbit polyclonal antiAR antibody (N-20, Santa Cruz Biotechnology) for 1 h at room temperature.

Techniques: In Vitro, Construct, Binding Assay, Luciferase, Activity Assay, Plasmid Preparation, Activation Assay, Expressing, Transfection

Journal: Cancer Research

Article Title: High Fidelity Patient-Derived Xenografts for Accelerating Prostate Cancer Discovery and Drug Development

doi: 10.1158/0008-5472.can-13-2921-t

Figure Lengend Snippet: Figure 2. Transplantable tumor lines retain the histolopathologic characteristics of their original patient tumor. Transplantable tumor lines LTL331 and LTL352 show similar tissue structure to the patient tumor from which they were originally derived (H&E stain). Immunohistochemical stains show that the protein expression of key markers (AR, PSA, PTEN, ERG, and SYP) is also conserved. LTL331, adenocarcinoma; LTL352, neuroendocrine prostate tumor. Scale bars, 100 mm.

Article Snippet: A rabbit polyclonal antiAR antibody (Affinity BioReagents), rabbit polyclonal anti-PSA antibody (Dako), rabbit monoclonal anti-PTEN antibody (Cell Signaling Technology) and rabbit monoclonal anti-ERG antibody (Epitomics) were used for immunohistochemistry.

Techniques: Derivative Assay, Staining, Immunohistochemical staining, Expressing